HALE STAIN FOR SIALIC ACID-CONTAINING MUCINS
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چکیده
منابع مشابه
Hale Stain for Sialic Acid-containing Mucins
The feasibility of using the Hale stain to identify cellular sialic acid-containing mucins by electron microscopy was investigated. Three kinds of mouse ascites tumor cells were fixed in neutral buffered formalin, exposed to fresh colloidal ferric oxide, treated with potassium ferrocyanide, imbedded in Selectron, and sectioned for electron microscopy. Additional staining with uranyl acetate and...
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Human epidermis, hair, nails, and kidney as well as bovine and horses' hooves were found to contain a lipid fraction, which on thin-layer chromatography migrated slightly ahead of the cerebroside sulfate esters and gave the color reaction specific for sialic acid. This fraction was isolated from horse hoof, in which it constituted nearly half of the total lipids. The purified fraction contained...
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The nature of the receptors for mucoid and nonmucoid Pseudomonas aeruginosa was investigated by using adherence to injured tracheal epithelium as a model. Bovine submaxillary mucin and crude rat tracheal mucin inhibited the adherence of both types of P. aeruginosa. Among the sugars present in these mucins only N-aceylneuraminic acid inhibited adherence. Inhibition of adherence probably involved...
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Mouse strain-specific differences in the carbohydrate composition of intestinal mucins were hypothesized to account for strain-dependent susceptibility to Entamoeba histolytica. To test this hypothesis, intestinal mucins from susceptible and resistant inbred strains of mice were analyzed for their O-glycan content and for their ability to inhibit amoebic adherence to (GalNAc)12-27-HSA neo-glyco...
متن کاملHALE STAIN FOR SIALIC A C I D - C O N T A I N I N G M U C I N S Adaptation to Electron Microscopy
T he fcasibility of using the Halc stain to identify cellular sialic acid-containing mucins by electron microscopy was investigated. Thrcc kinds of mouse ascites tumor cclls were fixed in neut ra l buffered formalin, exposed to fresh colloidal fcrric oxide, t reated with potassium fcrrocyanide, imbedded in Selectron, and sectioned for electron microscopy. Addi t ional s taining with uranyl acct...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1963
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.19.1.223